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Contents

FASP Protocol

FASP Template

Steps

Cell/Tissue Samples:

Purified Protein Samples:

Buffer Definitions

Ordering Information

Notes

Measuring peptide concentration in eluant

Concentration of the peptides can be estimated by UV spectrometer assuming that 0.1% solution of vertebrate proteins has at 280 nm an extinction of 1.1 absorbance units.

Reference

FASP paper (from the supplement of Nature Methods: 6(5) 2009)

Trypsin

Why you do not want to have any residual reducing agent (e.g. DTT) in your digestion buffer:

Trypsin with disulfide bonds

Porcine Trypsin [Green: Backbone, Red: Disulfide bonds]

In Gel FASP Protocol

Also, try our new and improved In-gel FASP method!