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Basic Protocol

For Running Lipid Standards:

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.



Lipidomics and lipid profiling in metabolomics. [2]

Lipidomics: coming to grips with lipid diversity, Nature Reviews Molecular Cell Biology, 11, 593–598 (1 August 2010).

Membrane lipid composition. [3]

Lipid rafts. [4]

Mass Spectrometry

Practical essentials of ESI.* [5]

Solid tutorial review on mass spec "-omics."* [6]

General approaches to lipidomics. [7]

Recent advances (2011) in MS lipidomics. [8]

Advances is mass spectrometry lipidomics. [9]

Specific lipid classes and MS. [10]

Basics of ESI and ESI intrasource separation shotgun lipidomics.** [11] [12]

Factors influencing ESI intrasource separation. [13]

Multiple precursor ion and neutral loss scanning with data-dependent acquisition. [16]

Top-down lipidomic strategy.** [17]

Top-down lipidomic analysis of blood plasma.* [18]

Quantitative shotgun yeast lipidomics. [19] [20]

Quantitative shotgun membrane lipidomics, including glycosphingolipids. [21]

LTQ Orbitrap shotgun lipidomics. [22]

Tandem Mass Spectrometry

Membrane lipids. [23]

Phospholipids [24] [25]

Sphingolipids [26]

Glycosphingolipids [27]


Informatics and computational strategies for the study of lipids. [28]

Computational lipidomics. [29]

Chemometrics for Metabonomics. [30] [31]

LipidXplorer: open-source lipid identification and quantification software. [32]

PCA package. [34]

K-OPLS package. [35]

S-plot. [36]

Special Journal Issues on Lipidomics

Journal of Chromatography B, Volume 877, Issue 26. [37]

Prostaglandins and Other Lipid Mediators, Volume 77. [38]

European Journal of Lipid Science and Technology, Volume 111, Issue 1. [39]

Frontiers in Bioscience, Volume 12, January 2007.

Methods in Enzymology, Volume 432, 2007.

Signalling Lipids and Lipidomics

Lipid rafts and GPCRs. [40]

Eicosanoid biology. [41]

Sphingolipid signalling. [42]

Lipid mediators in health and disease. [43]

Lipid mediators for inflammation resolution. [44]

Eicosanoid lipidomics. [45]

Bioactive lipidomics. [46]

Lipid mediator informatics-lipidomics. [47]

Mediator lipidomics. [48]

LC/MS Lipidomics

HPLC/MS of complex lipidomes. [49]

UPLC/MS of complex lipidomes. [50]


Mackay, 12/28/2011, with PE standard is solvent with ammonium acetate using positive-ion mode:

Group Members


Former or Associated

Relevant Publications

Error fetching PMID 19935646:
  1. Error fetching PMID 19935646: [P1]
    This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.